KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, Peptides, and Recombinant Proteins | ||
| L-glucose | Carbosynth | MG05247, CAS: 921-60-8 |
| all-trans-retinal | Sigma-Aldrich | R2500–100MG, CAS: 116-31-4 |
| Critical Commercial Assays | ||
| Pierce BCA Protein Assay Kit | Thermo Scientific | Cat. #23225 |
| Triglyceride LiquiColor Test (Enzymatic) | Stanbio | Ref. #2100–430 |
| Deposited Data | ||
| RNA sequencing | this paper | Accession #GSE113159 |
| Experimental Models: Organisms/Strains | ||
| Drosophila, w[1118]CS | A. Simon | n/a |
| Drosophila, y*plin2[51]/FM7i;P{w[+mc] = ActGFP}JMR3 or hom or Dp(1;Y) y[+] | R. Kühnlein | RKF610 |
| Drosophila, w*;bmm[1]/TM3,Sb[1] float | R. Kühnlein | SGF529 |
| Drosophila, w[*]; P{w[+mC] = Gr64f-GAL4.9.7}5/CyO | H. Amrein | BDSC #57669; Dahanukar et al., 2007 |
| Drosophila, w[*]; P{w[+mC] = Gr5a-GAL4.8.5}6; Dr[1]/TM3, Sb[1] | K. Scott | BDSC #57592; Chyb et al., 2003 |
| Drosophila, w[1118]; P{w[+mC] = UAS-GFP.nls}14 | Bloomington | BDSC #4775 |
| Drosophila, y[1] w[*]; P{w[+mC] = UAS-NaChBac}2 | M. Nitabach | BDSC #9469; Nitabach et al., 2006 |
| Drosophila, UAST-sxc(Ogt)RNAiCLb38 | C. Lehner | Radermacher et al., 2014 |
| Drosophila, y[1] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.HMJ03131}attP40 | Bloomington | BDSC #50909 |
| Drosophila, w[1118]; P{w[+mC] = UASGCaMP6s. brpS.mCherry}2 | Bloomington | BDSC #77131 |
| Drosophila, w[1118]; P{y[+t7.7] w[+mC] = 20XUAS-IVS-CsChrimson.mVenus}attP40 | Bloomington | BDSC #55135 |
| Oligonucleotides | ||
| Rp49 Forward, ATGCTAAGCTGTCGCACAAA | Integrated DNA Technologies | N/A |
| Rp49 Reverse, ACTTCTTGAATCCGGTGGGC | Integrated DNA Technologies | N/A |
| OGT Forward, CGTCCGCGGCCCATATATTA | Integrated DNA Technologies | N/A |
| OGT Reverse, CCAACTCGAGTAAACCGACTGA | Integrated DNA Technologies | N/A |
| Dilp2 Forward, TCAATCCCCTGCAGTTTGTC | Integrated DNA Technologies | N/A |
| Dilp2 Reverse, TTGAGTACACCCCCAAGATA | Integrated DNA Technologies | N/A |
| Dilp5 Forward, TCCTGATCCCGCTCCTGCTA | Integrated DNA Technologies | N/A |
| Dilp5 Reverse, TGCCTCGTTTGGCGAACATT | Integrated DNA Technologies | N/A |
| Software and Algorithms | ||
| Olympus FluoView FV1200-ASW 4.2 | Olympus Life Science | N/A |
| FLIC Monitor | https://sites.google.com/a/umich.edu/pletcher-lab/fly-tech | Ro et al., 2014 |
| RStudio | RStudio, Inc. | N/A |
| FLIC analysis R code | https://sites.google.com/a/umich.edu/pletcher-lab/fly-tech | Ro et al., 2014, this paper |
| Axon pCLAMP 9 Clampfit | Molecular Devices | N/A |
| Imaris | Bitplane | N/A |