Table 1. Bacterial strains and plasmids.
| Strains or Plasmids | Description | Genotype |
|---|---|---|
| Strains | ||
| MG1655 | E. coli K12 nonpathogenic | O Serogroup: N/A Pathotype: N/A Shiga toxin 1 (stx1): - Shiga toxin 2 (stx2): - Intimin (eae): - slp: + |
| O157-WT EDL 932 (ATCC #43894) |
E. coli O157:H7 wild-type |
O Serogroup: O157 Pathotype: EHEC Shiga toxin 1 (stx1): + Shiga toxin 2 (stx2): + Intimin (eae): + slp: + |
| O157-Δslp |
E. coli O157:H7 Δslp |
O Serogroup: O157 Pathotype: EHEC Shiga toxin 1 (stx1): + Shiga toxin 2 (stx2): + Intimin (eae): + slp: - |
| O157Δslp-p:slp |
E. coli O157:H7 Δslp + puc18::slp |
O Serogroup: O157 Pathotype: EHEC Shiga toxin 1 (stx1): + Shiga toxin 2 (stx2): + Intimin (eae): + slp: + |
| O26 |
E. coli O26 field isolate* |
O Serogroup: O26 Pathotype: EHEC Shiga toxin 1 (stx1): + Shiga toxin 2 (stx2): + Intimin (eae): + slp: + |
| O103 | E. coli O103 field isolate* | O Serogroup: O103 Pathotype: STEC Shiga toxin 1 (stx1): + Shiga toxin 2 (stx2): + Intimin (eae): - slp: + |
| O145 | E. coli O145 field isolate* | O Serogroup: O145 Pathotype: EPEC Shiga toxin 1 (stx1): - Shiga toxin 2 (stx2): - Intimin (eae): + slp: + |
| Plasmids | ||
| pKD119 | λ Red recombinase expression plasmid | Antibiotic resistance: Tetracycline (TetR) Growth: 30°C |
| pKD3 | λ Red recombinase plasmid containing FRT-flanked chloramphenicol resistance cassette | Antibiotic resistance: Chloramphenicol (CmR) |
| pCP20 | λ Red recombinase FLP expression plasmid | Antibiotic resistance: Ampicillin (AmpR) Growth: 30°C |
| pUC18 | Complementation cloning plasmid | Antibiotic resistance: Ampicillin (AmpR) |
*Isolates were provided by Dr. Chitrita DebRoy at the E. coli Reference Center at the Pennsylvania State University, University Park, PA.