Table 2. Primer sequences used in this study.
| Gene | Reference Sequence | Purpose | Sequence 5’-3’ |
|---|---|---|---|
| eae | NC_002695.1 | qPCR |
F:GTCGTGTCTGCTAAAACCGC R:CGGCGGAACTGGAAGTTAGT |
| gapA | NC_002695.1 | qPCR |
F:ACTTCGACAAATATGCTGGC R:CGGGATGATGTTCTGGGAA |
| slp | NC_002695.1 | qPCR |
F:GTTACCATCCTCGGCACCAT R:CAAATGCCACACCTGGATGC |
| slp | NC_002695.1 | Mutation |
F:GTGCTGCTAATGCGGATGCGACYTTCAAGGTTCAGTGTGTAGGCTGGAGCTGCTTC R:TTACTGATAGGTTAAAGAGAACCAGGCCTGTGCATTCATATGAATATCCTCCTTAG |
| slp | NC_002695.1 | Screening |
F:AGGTGCACTCATACTCAGCC R:TGCACCATAGCCGTAATCCC |
| slp | NC_002695.1 | Sequencing |
F:TCGCCTCAGAATCAGATGAAA R:ATCTGCATCTTTCGGTGGTG |
| slp | NC_002695.1 | Complementation |
F:TAAGCAAAGCTTATGGTTTTAATATTTGTTGATAAG R:TGCTTAGAATTCTTATTTGACCAGCTCAGGTGTTAC |