Fig 6. 3D-PCR analysis for detection of APOBEC3 activity.

(A-C) 3D-PCR products (170 bp) amplified from DMSO- (A), CDM-3008- (B), and IFNα- (C) treated PXB cells at different denaturation temperatures (80–92 °C) were separated by 2% agarose gel electrophoresis and visualized. (D) The 3D-PCR products obtained at a denaturing temperature of 91.2 °C were sequenced, aligned with the consensus sequence, and numbered. Mutations are shown as inverted letters.