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. 2019 Jun 12;14(6):e0218197. doi: 10.1371/journal.pone.0218197

Fig 2. Micropatterned cells show subcellular alignment and peripheral aggregation of actin.

Fig 2

ECFCs and ECs were stained and imaged using laser scanning confocal microscopy with a super-resolution airyscan detector in order to assess actin distribution and alignment after 24 hours of culture. Representative images of non-patterned ECFCs (A), micropatterned ECFCs (B), non-patterned ECs (C), and micropatterned ECs (D) after 24 hours in culture are shown. Each image series shows phalloidin (actin), VE-Cadherin, and a pseudocolor merge with phalloidin in green, VE-Cadherin in red, and a DAPI nuclear stain in blue. Main panel scale bars are 20 μm. Inset panels are 10X maximum Z-projections, with scale bars of 100 μm.