Table 1. Final mathematically defined media recipe.

Chemical	Final concentration
Ammonium chloride	18.5 mM
Ammonium sulphate	23.7 mM
Potassium nitrate	19 mM
Sodium carbonate	0.63 mM
Sodium chloride	3.03 mM
Dipotassium hydrogen phosphate	33.6 mM
Sodium di-hydrogen phosphate	21.5 mM
Bis-Tris-HCl pH7	43.3 mM
Calcium chloride	0.91 mM
Iron sulphate	0.04 mM
Magnesium sulphate	0.59 mM
NTA sodium salt	1.05 mM
Biotin	19 μM
MEM vitamin solution (Thermo Scientific)	1 x
MEM amino acids (Thermo Scientific)	1 x
MEM non-essential amino acids (Thermo Scientific)	1 x
Trace metal solution (S3 Table)	1 x
Glucose	10 g L-1

Concentrations of the components for the best performing media formulation predicted by the ANN model from the second iteration. Note: the combination of the two phosphate salts give a final pH of 7.