Table I.

Quantitative analysis of uORF peptide and luciferase protein during in vitro translation

RNA	Relative Amount of uORF Peptidea	Relative Amount of Luciferasea	Molar Ratio of uORF Peptide to Luciferase
LUCWT	133 ± 10	100	1.3 ± 0.1
LUCcontxt	212 ± 17	59 ± 5	3.6 ± 0.5
LUCdm	265 ± 13	219 ± 12	1.2 ± 0.1
LUCdmcontxt	384 ± 27	103 ± 8	3.7 ± 0.3
LUCWTfus	–b	168 ± 8	–
LUCdmfus	–	327 ± 21	–

^a Values have been adjusted for the no. of labeled methionine residues in each product with the relative molar amount of luciferase in LUCWT set as 100. Each value (±sd) represents the average of at least four independent SDS-PAGE analyses. 

^b (–), Does not apply to this RNA.