Table I.
List of antibodies used in the present study.
| Antibody | Supplier | Cat. no. | Dilution |
|---|---|---|---|
| p-AKT 1/2/3 | Cell Signaling Technology, Inc. | 4060 | 1:1000 |
| AKT | 9272 | 1:1000 | |
| p-ERK 1/2 | 4370 | 1:1000 | |
| ERK | 4695 | 1:1000 | |
| Cleaved caspase-3 | 9664 | 1:1000 | |
| SDHA | 5839 | 1:1000 | |
| Cytochrome c | 4280 | 1:1000 | |
| COX4 | 4850 | 1:1000 | |
| ERO1 | 3264 | 1:1000 | |
| IRE1 | 3294 | 1:1000 | |
| HSP60 | 4871 | 1:1000 | |
| PARP | 9532 | 1:1000 | |
| γH2AX | Santa Cruz Biotechnology, Inc. | sc-101696 | 1:200 |
| H2AX | sc-54606 | 1:200 | |
| NDUFA1 | sc-376357 | 1:200 | |
| ATP5B | sc-166443 | 1:200 | |
| β-actin | Sigma Aldrich; Merck KGaA | A1978 | 1:2000 |
| IRDye 800 CW goat anti-rabbit | LI-COR Biosciences | 926-32211 | 1:5000 |
| IRDye 680 CW goat anti-mouse | 925-68070 | 1:5000 | |
| IRDye 800 CW donkey anti-goat | 926-32214 | 1:5000 |
p-, phosphorylated, AKT, protein kinase B; ERK, extracellular signal-regulated kinase; SDHA, succinate dehydrogenase flavoprotein subunit; COX4, cytochrome c oxidase subunit 4; ERO1, endoplasmic reticulum oxidoreductin-1; IRE1, inositol-requiring enzyme 1; HSP60, heat shock protein 60; PARP, poly [ADP-ribose] polymerase; H2AX, histone H2AX; γ, phosphorylated; NDUFA1, NADH dehydrogenase 1 α-1; ATP5B, ATP synthase subunit β.