Skip to main content
. Author manuscript; available in PMC: 2019 Jun 12.
Published in final edited form as: J Allergy Clin Immunol. 2018 May 30;142(3):997–1000.e4. doi: 10.1016/j.jaci.2018.04.038

FIG E2.

FIG E2.

Characterization of CD8+ T cells plasticity. A, CD8+ T cells from WT NOD and T143-ββ mice were purified and cultured with anti-CD3 and anti–CD28-coated beads in the presence of IL-2, TGFβ, and anti–IFN-γ to induce FoxP3 expression. Cells were analyzed after 4 days in culture. Representative flow cytometric analysis (left) and scatter plot (right) are shown. B, CD8+ T cells from WT NOD and T143-ββ mice were purified and cultured with IL-2, IL-12, and anti–IL-4 to induce TH1 differentiation. Cells were analyzed after 4 days in culture. Representative flow cytometric analysis (left) and scatter plot (right) are shown. n.s., Not significant. Error bars are expressed as mean ± SEM; ***P < .001.