P311 was induced during 3T3-L1 adipogenesis.
A. A qPCR study was used to examine the expression of P311. B. Western blot analysis was performed to verify P311 protein expression. The lower panel shows the loading control, GAPDH. C & D. Expression of the key adipogenic markers PPARγ2 and aP2. E - H. Gene expression profile of 3T3-L1 adipogenesis. qPCR was used to examine the expression of P311 (p<0.0001) (E) and the adipogenic markers PPARγ2 (p<0.001) (F), C/EBPβ (p<0.0001) (G) and C/EBPα (p<0.0001) (H) in 3T3-L1 preadipocytes differentiated for different days (D0 to D10).
