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. Author manuscript; available in PMC: 2020 Jul 12.
Published in final edited form as: Biochem Biophys Res Commun. 2019 May 27;515(1):234–240. doi: 10.1016/j.bbrc.2019.05.105

P311 is a nuclear and cytoplasmic protein.

P311 is a nuclear and cytoplasmic protein.

A. In pcP311 transfected 3T3-L1 preadipocytes, c-myc-tagged-P311 (green) were mainly localized in the cytoplasm (red arrows). Blue stain (red arrow heads) indicates the DAPI stain for nuclei. In a small population of 3T3-L1 preadipocytes, P311 was translocated to the nucleus (top right panel, white arrows) upon adipogenic induction. Differentiated mature 3T3-L1 adipocytes showed P311 in both the nuclei (aqua green, white arrow heads) and cytoplasm (green, red arrows), as tested with anti-c-myc tag antibody. Empty vector (pCMV) transfected 3T3-L1 adipocytes served as control. B. Differentiated mature 3T3-L1 adipocytes showed P311 in both the nucleus (aqua green, white arrow heads) and cytoplasm (green, red arrows), as tested with two anti-P311 antibodies.