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. 2019 Apr 22;2019:3503912. doi: 10.1155/2019/3503912

Figure 3.

Figure 3

Effects of auranofin on RANKL-activated IKK/IκBα–dependent NF-κB activation. (a) Relative activity of the NF-κB reporter gene in 100 ng/mL RANKL-exposed Raw264.7 cells (n = 6). (b) Effect of auranofin on c-Fos expression in BMMs (n = 3). (c, d) Effect of auranofin on nuclear translocation of p65. BMMs were preincubated with 3 μM auranofin for 15 min and exposed to 100 ng/mL RANKL for the indicated time points. Nuclear translocation of p65 was visualized by immunoblottings using subcellular fractionation (c) and immunocytochemistry (d, red arrows indicate nuclear p65). (e, f) Effects of auranofin on phosphorylation of IKKα/β and IκBα in RANKL-stimulated BMMs. P < 0.05 compared with the control group; # P < 0.05 and ### P < 0.005 compared with the RANKL-treated group.