Table 3. Cryo-EM AAV2-PKD1-2 data collection and processing statistics.
| Data collection: | |
|---|---|
| Magnification | 75,000 x |
| Voltage | 300 kV |
| Electron exposure | 25 e¯/Ų |
| Defocus range | −0.8 to −2.0 μm |
| Pixel size | 1.049 Å |
| Data processing: | |
| Motion correction | Motioncor2 1.1.0 |
| Anisotropic magnification correction: | |
| Distortion angle | 3.2° |
| Percent distortion | 1.10% |
| CTF estimation | Gctf 1.06 |
| Resolution range | 30 to 3 Å |
| Symmetry imposed | I1 |
| Initial particle images | 34,450 |
| Final particle images | 21,373 |
| Map resolution | 2.39 Å |
| FSC threshold | 0.143 |