Key resources table.
Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Genetic reagent (M. musculus) | Foxp2Fx (Foxp2tm1.1Sfis) | French et al., 2007 | MGI Cat# 3800702, RRID:MGI:3800702 | |
Genetic reagent (M. musculus) | Rosa-TdTomato (Ai14) | Jackson Laboratory | IMSR Cat# JAX:007914, RRID:IMSR_JAX:007914 | |
Genetic reagent (M. musculus) | Ntsr1-cre (GN220) | MMRRC | MMRRC Cat# 030648-UCD, RRID:MMRRC_030648-UCD | |
Genetic reagent (M. musculus) | Emx1-cre | Gorski et al., 2002 | IMSR Cat# JAX:005628, RRID:IMSR_JAX:005628 | |
Genetic reagent (M. musculus) | MetEGFP BAC (MetGFP) | Gong et al., 2003 | MGI:6144427 | |
Antibody | Goat anti-Foxp2 (polyclonal) | Santa Cruz Biotechnology | Cat# sc-21069, RRID:AB_2107124 | IHC (1:100) |
Antibody | Chicken anti-GFP (polyclonal) | Abcam | Cat# ab13970, RRID:AB_300798 | IHC (1:500) |
Antibody | Rat anti-Ctip2 (monoclonal) | Abcam | Cat# ab18465, RRID:AB_2064130 | IHC (1:500) |
Antibody | Guinea Pig anti-ppCCK | Watakabe et al., 2012 | Dr. Takeshi Kaneko (University of Tokyo) | IHC (1:500) |
Antibody | Rabbit anti-PCP4(PEP-19) | Dr. James Morgan (St. Jude's Research Hospital) | IHC (1:3000) | |
Antibody | Rabbit anti-Fog2 (polyclonal) | Santa Cruz Biotechnology | Cat# sc-10755, RRID:AB_2218978 | IHC (1:250) |
Antibody | Rabbit anti-DARPP-32 (monoclonal) | Cell Signaling Technology | Cat# 2306, RRID:AB_823479 | IHC (1:500) |
Antibody | AlexaFluor F(AB')2 488- or 594- or 647- secondaries | Jackson Immunoresearch Laboratories, Inc | IHC (1:500) | |
Peptide, recombinant protein | Cholera Toxin Subunit B (CTB) | Invitrogen | Cat. #: C-34776, C-34778 | Alexa Fluor Conjugate (555, 647) |
Commercial assay or kit | BaseScope assay | Advanced Cell Diagnostics | Cat. #: 323971 | |
Software, algorithm | IMARIS | Imaris (http://www.bitplane.com/imaris/imaris) | RRID:SCR_007370 | Microscopy Image Analysis Software |
Software, algorithm | Adobe Photoshop | Adobe Photoshop (https://www.adobe.com/products/photoshop.html) | RRID:SCR_014199 | |
Software, algorithm | Adobe Illustrator (CS6) | Adobe Illustrator (http://www.adobe.com/products/illustrator.html) | RRID:SCR_010279 | |
Software, algorithm | Code used for nuclear immunofluorescent quantification | This paper | custom written for ImageJ macros (Source Code 1; Source Code 2) | |
Software, algorithm | ImageJ | ImageJ (http://imagej.nih.gov/ij/) | RRID:SCR_003070 | |
Software, algorithm | GraphPad Prism 6 | GraphPad Prism (https://graphpad.com) | RRID:SCR_015807 | Version 6 |