Skip to main content
. 2019 Jun 12;5(6):eaaw4991. doi: 10.1126/sciadv.aaw4991

Fig. 3. AdOPG does not affect hMSC mineralization on Col-GAG or MC-GAG.

Fig. 3

qRT-PCR of control or AdOPG-transduced primary hMSCs cultured on Col-GAG or MC-GAG materials for 14 days in osteogenic differentiation medium for (A) RUNX2 and (B) OPN (n = 3). Western blot of (C) control or (D) AdOPG-transduced primary hMSCs cultured on Col-GAG or MC-GAG materials for 56 days in osteogenic differentiation medium for Smad5 and p-Smad1/5. (E) Representative μCT images and (F) quantitative analysis of control or AdOPG-transduced primary hMSCs cultured on Col-GAG or MC-GAG for 8 weeks (n = 3). Bars represent means, and error bars represent SD. Significant post hoc comparisons following ANOVA indicated with P values.