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. 2019 Jun 12;5(6):eaaw4991. doi: 10.1126/sciadv.aaw4991

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Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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Fig. 5 — (A) WST-1 proliferation and viability assays of primary pre-osteoclasts in single culture (OC only), cocultured with empty scaffolds, or cocultured with control or AdOPG-transduced hMSCs (control hMSC and AdOPG hMSC, respectively) in medium supplemented with RANKL and M-CSF on Col-GAG or MC-GAG for 14 days. (B) TRAP staining (top row), resorption pits (middle row), and live images (bottom row) of negative control without cells (no cells), osteoclast only without hMSCs or scaffolds (OC only), and osteoclasts cocultured with Col-GAG or MC-GAG as empty scaffolds (empty scaffold), with control hMSCs (control), or with AdOPG-transduced hMSCs (AdOPG). (C) Quantitative analysis of pit assays as percentage of total area of well in differentiated osteoclasts without hMSCs (OC only) and osteoclasts cocultured with Col-GAG and MC-GAG as empty scaffolds, scaffolds with control hMSCs, and scaffolds with AdOPG-transduced hMSCs. Significant post hoc comparisons following ANOVA indicated with P values.