Fig. 2.

GnRH expression is suppressed by LIF. a GT1–7 cells transiently transfected with the 5-kb GnRH reporter (5-kb GnRH Luc) treated with vehicle (CTR, white bar), or TNF-α, IL-1β, IL-6, and LIF, for 24 h. Luciferase values were normalized to β-galactosidase values for each sample. Results are presented as an average of three experiments performed in triplicate. Statistical significance (p < 0.05) was determined with ANOVA followed by Tukey’s post hoc test and indicated with an asterisk. b RT-qPCR using total RNA from GT1–7 cells treated with LIF, 10 ng/mL for 24 h, demonstrates repression of endogenous GnRH mRNA (Gnrh1). Asterisk indicates significant difference determined with the t test (p < 0.05).