Figure 2. Effects of a rex mutation on expression of NAD⁺-regeneration genes.

Expression of the prdA (A), CD2344 (B), bcd2 (C), and grdE (D) genes was measured by qRT-PCR in wild- type, rex mutant and complemented rex mutant strains. Cells were grown in TY medium with or without supplementation with 30 mM L-proline to mid-exponential phase as described under Experimental procedures. The means and standard error of the means of at least three biological replicates are shown.