Table 5.
Strains and plasmids used in this study
| Characteristics | Reference or source | |
|---|---|---|
| Strain | ||
| E. coli | ||
| HB101 | F–supE44 hsdS20(r-Bm-B) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 | |
| DH5α | F– Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rK–, mK+) phoA supE44 λ– thi-1 gyrA96 relA1 | |
| C. difficile | ||
| JIR8094 | EmS derivative of strain 630 | (O’Connor et al., 2006) |
| LB-CD8 | JIR8094 prdR::ermB | (Bouillaut et al., 2013) |
| LB-CD24 | JIR8094 rex::ermB | This study |
| ND-CD23 | JIR8094 rex::ermB Tn916::rex | This study |
| B. subtilis | ||
| BS49 | (Manganelli et al., 1998) | |
|
Plasmid | ||
| pBL26 | pSMB47 containing catP | This study |
| pBL64 | pCR2.1-intron template part A | (Bouillaut et al., 2013) |
| pBL65 | pCR2.1-intron template part B | (Bouillaut et al., 2013) |
| pBL100 | pBL68 containing un-targeted groupII intron – Targetron vector | (Bouillaut et al., 2013) |
| pBL130 | pBL100 targeted to rex | This study |
| pCR2.1 | Invitrogen® | |
| pND1 | pBL26 containing rex gene with its own promoter | This study |
| pRK24 | Tra+, Mob+, AmpR, TcR | (Trieu-Cuot et al., 1991) |
| pSMB47 | (Manganelli et al., 1998) | |