Table 2.
Substrate scope of Mb(H64V,I107S) and Mb(L29A,H64V,V68A).a
 | ||||
|---|---|---|---|---|
| Entry | Product | Catalyst | Yieldb | e.e. |
| 1 |  |
a b |
99% 95% |
99% 96% |
| 2 |  |
a b |
83% 66% |
97% 89% |
| 3 |  |
a b |
99% 85% |
94% 80% |
| 4 |  |
a b |
62% 45% |
95% 68% |
| 5 |  |
a b |
90% 55% |
96% 70% |
| 6 |  |
a b |
77% 82% |
90% 39% |
| 7 |  |
a b |
95% 70% |
99% 74% |
| 8 |  |
a b |
99% 62% |
99% 68% |
| 9 |  |
a b |
65% 23% |
97% 81% |
| 10 |  |
a b |
71% 95% |
79% 97% |
| 11 |  |
a b |
99% 99% |
10% 52% |
| 12 |  |
a b |
87% 89% |
5% 38% |
| 13 |  |
a b |
43% 68% |
74% 51% |
a
Reaction conditions: 2.5 mM diazoacetate, Mb-expressing E. coli (OD600 = 40) in KPi buffer (50 mM, pH 7), 40 mL-scale, r.t., 3–5 hours.
b
GC or SFC yield. See Table S5 for additional data.