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. 2019 Jan 17;26(5):e12520. doi: 10.1111/micc.12520

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© 2018 John Wiley & Sons Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Both endothelial cells and pericytes share markers used in the literature for labeling endothelial cells, and Col‐IV tracks, assumed to be regressed vessels, lack a pixel intensity profile indicative of a lumen. (A) Retina capillary with pericyte (NG2, red), IB4 lectin (green), endothelial cells (CD31, yellow), and cell nuclei (DAPI, cyan). (B) Retina capillary with pericyte and endothelial cells labeled with Col‐IV (green; scale bar 10 μm). (C) High‐resolution image of Col‐IV off‐vessel track (star) and lumenized blood vessel (arrow; scale bar 5 μm). (D) Comparison of Col‐IV relative pixel intensity profile across cross‐section of blood vessels and collagen tracks (P = 8.11E‐6, 2‐way analysis of variance, N = 10 vessels and tracks, error bars are standard deviation)