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. 2019 Apr 2;100(6):1461–1472. doi: 10.1093/biolre/ioz052

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© The Author(s) 2019. Published by Oxford University Press on behalf of Society for the Study of Reproduction.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — The localization of PERF15 in the perinuclear theca of rat spermatozoa. (A) Immunolocalization with an affinity-purified anti-PERF15 antibody at the ultrastructural and fluorescent levels. Cross-sectional electron micrographs localize PERF15 immunoreactivity to the triangular perforatorium as well as ventral spur (VS). Immunofluorescence appears to dominate the apical perforatorial hook (bottom right). (B) Diagrammatic representation of the pattern of PERF15 labeling in mature rat spermatozoa. A, acrosome; IAM, inner acrosomal membrane; OAM, outer acrosomal membrane; ES, equatorial segment; HCS, displaced head cap segment; PM, plasma membrane; N, nucleus; P, perforatorium.