Figure 6.

Native PAGE/activity gels (zymograms) of starch modifying activities. A, Twenty-five micrograms of total protein from Su1 and su1-R4582::Mu1 homozygous kernels harvested at 20 DAP was separated on a 5-cm native polyacrylamide gel, then electroblotted to a starch-containing gel. Starch modifying enzyme activities were visualized by staining with I₂/KI. B, Fifty or 100 μg total protein from Su1, su1-R4582::Mu1, su1-st, and su1-Ref homozygous kernels harvested at 20 DAP was analyzed as described for A on 15-cm native PAGE/activity gels. Arrows A through G indicate starch modifying activities that exhibit allele-specific variation.