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Purification scheme for bovine lactoperoxidase.
| Purification Step | Total protein (mg) | Total activity (units)* | S.A.(units/mg protein) | Fold purification | Recovery % |
|---|---|---|---|---|---|
| skimmed milk homogenate | 210 | 1197 | 5.7 | — | 100 |
| Mono S 5/50 GL column | 40 | 812 | 20.3 | 3.56 | 67.8 |
| Sephacryl S-100 column | 12 | 546 | 45.5 | 7.98 | 45.6 |
*One unit of lactoperoxidase was defined as the increasing in the absorption as a result of the formation of the oxidized ABTS.
