Table 2.

Characterization of TDP–TTX conjugates

Conjugate	Feed dicarboxylic acid (mmol)	Feed triol (mmol)	Feed PEG (mmol)	Feed TTX (mmol)	TTX loadinga (µg mg−1)	Proportion of bound TTXb (wt%)
TgD8P2000–TTX	5	1.25	2.5	0.003	0.16	99.2
TgD8P1000–TTX	5	1.25	2.5	0.003	0.28	99.0
TgD8P200–TTX	10	2.5	5	0.003	0.31	99.5
TgD8–TTX	10	5.8	–	0.003	0.40	99.4
TgD8–TTXHc	10	5.8	–	0.012	1.60	99.4
TgD5–TTX	10	5.8	–	0.003	0.54	99.5
TgD1–TTX	10	5.8	–	0.003	0.64	99.4
TcD8–TTX	10	5.8	–	0.003	0.27	99.6

^aAs determined by ELISA

^bProportion of TTX conjugated with polymer as a fraction of the total TTX in the reaction

^cT_gD₈–TTX_H has four-fold higher TTX loading than T_gD₈–TTX