Table 3.
Myotoxicity and inflammation
| Formulation | Dose of polymer (mg) | Dose of TTX (µg) | Myotoxicity score (range) Day 4 |
Myotoxicity score (range) Day 14 |
Inflammation score (range) Day 4 |
Inflammation score (range) Day 14 |
|---|---|---|---|---|---|---|
| Free TTXa | – | 3.0 | 0 (0–1.0) | 0 (0–1.0) | 0 (0–1.0) | 0 (0–1.0) |
| PEG200 | 562b | 3.0 | 0 (0–1.0) | 0 (0–1.0) | 0 (0–1.0) | 1.0 (0–1.0) |
| p Value | 1.000 | 1.000 | 1.000 | 0.456 | ||
| TgD8–TTXH | 50c | 80.0 | 1.0 (0–1.0) | 1.0 (0–1.0) | 1.0 (0–1.0) | 1.0 (0–1.0) |
| p Value | 0.456 | 0.456 | 0.456 | 0.456 | ||
| TgD1–TTX | 12.5c | 31.9 | 1.0 (0–1.0) | 1.0 (0–1.0) | 0 (0–1.0) | 1.0 (0–1.0) |
| p Value | 0.456 | 1.000 | 1.000 | 0.456 | ||
| TgD8P200–TTX | 32.5c | 10.0 | 0 (0–1.0) | 0 (0–1.0) | 1.0 (0–1.0) | 0 (0–1.0) |
| p Value | 1.000 | 0.456 | 0.456 | 1.000 | ||
| TgD8P1000–TTX | 25c | 6.9 | 0 (0-0) | 0.5 (0–1.0) | 0.5 (0–1.0) | 0.5 (0–1.0) |
| p Value | 0.414 | 0.738 | 0.738 | 0.738 | ||
| TgD8P2000–TTX | 25c | 3.5 | 0.5 (0–1.0) | 0 (0–0) | 0.5 (0–1.0) | 0.5 (0–1.0) |
| p Value | 0.738 | 0.182 | 0.738 | 0.738 |
Data are medians with interquartile ranges. P values are for the comparison of the tissue reaction of test compounds to that of free TTX. n = 3 per experimental group. Inflammation scores range: 0–4; myotoxicity scores range: 0–6
a3 µg of free TTX in 0.5 mL of PBS
b3 µg of free TTX in 0.5 mL of PEG200
cTD–TTX and TDP–TTX conjugates were formulated in 0.5 mL of PEG200