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. 2019 Jun 12;10(6):461. doi: 10.1038/s41419-019-1713-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b Immunofluorescence microscopy of LPS-primed macrophages untreated or stimulated with ATP and then stained for ASC and DNA (with DAPI). Scale bars: 20 μm. c Percentage of macrophages containing ASC foci. The quantification represents the mean of three independent experiments, with at least 50 cells counted in each experiment. d, e ASC oligomerization and redistribution assay in peritoneal macrophages treated as in a and b. Immunoblot analysis of ASC in cross-linked pellets (upper panels) and in cell lysates (lower panels)