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. 2019 Jun 12;10(6):460. doi: 10.1038/s41419-019-1700-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Representative western blot images of total lysates extracted from human colon cancer and matched adjacent normal tissues (normalized to β-actin). b, c Quantitative immunoblot analysis of TRPV4 protein level in colon cancer tissues and matched normal control from 18 subjects. d Representative images of TRPV4 protein expression in colon cancer tissue and matched adjacent normal tissue by immunohistochemistry. e TRPV4 expression scores were displayed in scatter plot. f Kaplan–Meier plots of colon cancer patients with high and low TRPV4 expression. All quantitative data shown represent the means ± SEM of at least three independent experiments. ^*P < 0.05, ^$P < 0.01 and ^#P < 0.001, versus the adjacent normal group (for b)