Fig. 2. Functional TRPV4 channels are present in colon cancer cells.

RT-PCR analysis of TRPV4 mRNA expression (a) and western blot analysis of TRPV4 protein expression (b) in indicated colon cancer cells. β-actin was used as the loading control. c, d Representative images and summary data from intracellular Ca²⁺ measurement in response to 100 nM GSK1016790A (agonist, arrowhead) in HCT-116, HT-29, SW480 and SW620 cells that were pretreated with vehicle (0.1% DMSO) or HC-067047 (4 μΜ). e Summary data from intracellular Ca²⁺ measurement in response to 100 nM GSK1016790A in HCT-116, HT-29, SW480 and SW620 cells that were transfected with control siRNA (siCTL) or TRPV4 siRNA (siTRPV4#1). All quantitative data shown represent the means ± SEM of at least three independent experiments. ^#P < 0.001, versus vehicle treatment only (d) or the siCTL group (e)