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. 2019 Jun 12;10:2562. doi: 10.1038/s41467-019-10544-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Expression pattern of OsCd1 in rice. a Tissue-dependent expression of OsCd1 at vegetative stage. Left: GUS histochemical assay of the transgenic plant with the GUS reporter driven by the OsCd1 promoter. Right: the expression level of OsCd1 in root and shoot with and without 1 μM CdCl₂ treatment via qPCR. Bars = 2 mm. b Tissue-specific localization of OsCd1 in the root of rice using the GUS reporter driven by the OsCd1 promoter. Bars = 0.5 mm. c Tissue-specific localization of OsCd1 in the root of rice using the GFP driven by the OsCd1 promoter in cross frozen sections. Nip, negative control; Ubi::GFP, positive control. Bars = 10 μm. d The GFP transcriptional level shown by RT-PCR. Upper, GFP; Down, Actin. e Subcellular location of OsCd1 in the root of rice observed by confocal laser scanning microscopy. The GFP fluorescence, fluorescence of FM4-64 and overlay of FM4-64 and pMDC45-GFP (left), OsCd1-GFP (right) were shown, respectively. Bars = 20 μm. f Subcellular location of OsCd1 in root protoplasts. Bars = 10 μm. Source data of Fig. 3a and d are provided as a Source Data file