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. 2019 Jun 12;9:8564. doi: 10.1038/s41598-019-45089-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Age-dependent Dectin-1 protein expression and Gene Ontology analysis of sham whole blood. (a–d) Flow cytometry analysis showed reduced dectin-1 protein expression on Ly6G+ neutrophils in juvenile peripheral blood and spleen compared to adult. (e–g) Infiltrating CD45+ cells in the lesion of the adult brain also express dectin-1. (h–j) Dectin-1 expression is decreased in infiltrating CD45+ cells in the lesion of the juvenile brain. (k,l) Enrichr GO Biological Process analysis of significantly downregulated genes in juvenile vs adult sham peripheral blood. (m,n) KEGG pathway analysis of significantly downregulated genes in juvenile vs adult sham peripheral blood. P values were calculated using the hypergeometric method.