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. 2019 Jun 12;9:8553. doi: 10.1038/s41598-019-44908-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of the AIDA on cell cycle progression. The effect of expressing the VH/VL-proCASP3 proteins and their variants on the cell cycle was assessed by flow cytometry. Cells were induced for 48 hours, fixed, permeabilized, and stained with 7-AAD. Live, single cells were gated according to their size and granularity by forward and side scatter parameters. The normal cell cycle populations (G0/G1, S and G2/M phases) were set on the uninduced Parental cell line. Data are represented as overlaid histograms, with the cell cycle gates shown on each plot. The regions for subG1, G1, S and G2 + M phases are indicated. Blue trace = uninduced cells Red trace = tetracycline induced cells.