Fig. 1.

Clonal mutations detected in paired samples and used to monitor serial ctDNA from patients who received chemotherapy plus trastuzumab. (A) Relationship between clonal mutations in baseline ctDNA and matched tissues. (B) Patient counts and fractions of detected TP53 mutation in different studies. (C) The mTBI of ctDNA before treatment and during progressive disease. Top: Tumor burden changed based on baseline status. The gray line indicates CT imaging results. The red line indicates ΔmTBI results. Progressive metastases are marked in red font at PD. Bottom: serial changes of CNV detected in ctDNA. Patient P07 received palliative surgery with continuous evaluation of PR. BL, baseline; PR, partial response; PD, progressive disease; NA, no available CT result; PS, palliative surgery; mTBI, molecular tumor burden index; CT, computed tomography. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)