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. 2019 Jun 12;9:8531. doi: 10.1038/s41598-019-44630-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of Parasite load in spleen and liver in course of treatment with Vit.D₃/RA, CDCA/RA L. donovani infection in BALB/c mice in comparison of control. (A) Parasite load in spleen (B) Parasite load in the liver (C) Giemsa stained micrograph showing macrophages (spleen and liver) infected with L. donovani showing intracellular parasite converted to amastigotes after internalization (100X; indicated by arrowheads). All the treated and control groups mice were sacrificed and Leishman-Donovan units (LDU) were calculated from liver and spleen impression smears. The values represent the mean ± SD difference between the infected and control of experiments. *P < 0.05. **P < 0.005, n = 6.