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. 2019 Jun 12;9:8577. doi: 10.1038/s41598-019-44111-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Time-dependent NIR-ASM spectral changes initiated by the addition of NQO1 (2.5 µg/mL). (b) Time-dependent fluorescent intensity changes for the probe NIR-ASM in the presence of different concentrations of NQO1 (0 to 25 µM). Experiments were performed in triplicate and averages were plotted. (c) Kinetics plot of NQO1 (2 µg) towards NIR-ASM probe. Values shown are the average of 6 experiments. Assays were performed in 96 well plates at ex/em = 460/646 nm in the presence of 100 μM NADH. (d) Fluorescence response of NIR-ASM probe to various biologically relevant substrates. (e) Effect of NQO1 inhibitor ES936 on NQO1 activation of NIR-ASM. All experiments were conducted with 10 mM NIR-ASM and 100 mM NADH in 0.1% BSA containing PBS (pH 7.4) at λ_ex/em = 460/646 nm.