Fig. 3.

The SynMICdb Score. a Nine parameters are integrated into the SynMICdb score for the estimated impact of a synonymous mutation. b Synonymous mutations in known cancer genes rank higher in the SynMICdb score than mutations in other genes. Depicted is the fraction of synonymous mutations in cancer genes (red) vs. no cancer genes (blue) in 10% bins of the SynMICdb score ranking (the cancer gene parameter was excluded from the score). A 10% frequency would indicate equal distribution along the 10 bins of the SynMICdb score ranks. Comparing the score rank averages between the group of non-cancer genes and cancer genes revealed a highly significant difference (t-test, p < 0.001). c The average SynMICdb score is depicted along the length of the coding sequence in 10% bins. Significance: *p < 0.05, **p < 0.01, **p < 0.001 (t-test). d The average SynMICdb score is depicted along the length of the internal exons in 10% bins. Significance: *p < 0.05, **p < 0.001 (t-test). b–d Bars represent the difference from the average (x-axis) and the whiskers indicate the standard error (SEM). e The SynMICdb score, as well as multiple of its individual parameters are compared for 78,278 synonymous mutations falling into annotated cassette exons vs. all other synonymous mutations with the significance (log10 p-value t-test, left) and relative difference (% difference of average between both groups, right) depicted