Figure 5.

Influence of Esi47 on the promoter activities of the plant hormone-inducible genes in barley aleurone. Barley aleurone layers were bombarded with the gene constructs indicated. The effector constructs were Act1-Esi47 (the Esi47 protein kinase-coding region controlled by the promoter and 5′-UTR intron of the rice actin-1 gene Act1) and Act1-Esi47^K124Q (same as Act1-Esi47, but Esi47 contains the K124Q mutation). A, The barley ABA-inducible Hva1 gene promoter was fused to the reporter gene for GUS to form the reporter construct Hva1-GUS and the bombarded tissues were treated with 20 μm ABA for 24 h as indicated. B, The barley GA-inducible α-amylase gene promoter was fused to the reporter gene for GUS to form the reporter construct Amy-GUS and the bombarded tissues were treated with 1 μm GA for 24 h as indicated. In both experiments the controls were not treated with ABA or GA. In all assays GUS activities were measured and normalized against the luciferase activities cobombarded into the barley aleurone. The values are averages of four independent shootings and the sds are shown as error bars. The induction rates by ABA or GA are also shown.