JM1 Cells Release DAMPs and ROS When Treated with LTX-401
(A) JM1 cells release HMGB1 from the lysate (L) to supernatant (S) after being stimulated with 108 μM LTX-401. (B) ATP is released from JM1 cells into supernatant following treatment with 54 μM LTX-401. (C) Release of cytochrome c into supernatant of LTX-401-treated JM1 cells (108 μM). (D) JM1 cells release ROS after being treated with 271 μM LTX-401 for 45 min. Data are expressed as fold-change in ROS release relative to control. For (B)–(D), data depict the mean ± SEM. *p < 0.05, **p < 0.01, ****p < 0.0001 by Kruskal-Wallis test (B and C) and Student’s t test (D).