Figure 8.

Histopathological Examinations of JM1 Tumors

Representative images of tumor area from subcutaneous and intrahepatic localized tumors, taken from both control animals (Ctrl) and animals treated with LTX-401 (7 days post-treatment). (A–D) Sections stained with hematoxylin, eosin, and saffron (HES). (B and D) LTX-401-treated tumors displayed large areas of necrotic tumor tissue with small remaining nodules of vital tumor tissue. (Q and R) Masson-trichrome staining (M-T) of the liver tumors showed no fibrosis in control tumors and only low levels of collagen at the border of the necrotic tissue in LTX-401-treated animals. (E–H) Sections immunostained for CD3⁺ T cells, (I–L) CD8⁺ T cells, and (M–P) natural killer cells. Arrow points at border of tumor. (A–D, Q, and R) Original magnification ×20; scale bars, 500 μm. Insets in upper right corner show higher original magnification of the tumors: ×400. (E–P) Original magnification ×200; scale bars, 50 μm. gt, granulation tissue; n, necrosis; T, tumor.