Figure 2.

The relative expression of tight junction protein genes in the jejunum (a) and ileum (b) of goats fed hay diet and high‐grain (HG) diet (means ± SD, n = 5). *0.01 < p < 0.05, **0.001 < p < 0.01, ***p < 0.001. NS, not significant. The 18S rRNA (a housekeeping gene) mRNA level was used to normalize the relative amount of each studied mRNA, and the $2^{-\mathrm{\Delta }\mathrm{\Delta }\mathrm{C}\mathrm{T}}$ method was used to analyze the data. The data were analyzed by an independent t test.