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. 2019 May 27;16:192–205. doi: 10.1016/j.isci.2019.05.032

Figure 5.

Figure 5

MODY1 hiPSC-Derived Cells Express Both WT and Mutant HNF4A Transcripts

(A–C) Allele-specific qPCR analyses in (A) D14 HPPs, (B) D8 hepatoblasts, and (C) D35 β-like cells evaluating both WT and mutant HNF4A transcripts in MODY1-derived cells. Axes show relative fluorescence units for each allele-specific TaqMan probe, for a representative differentiation experiment.

(D) Luciferase assays were performed to evaluate effects of WT and mutant HNF4A in combination with AFP enhancer activity in HepG2 cells.

(E) Luciferase assays were performed to evaluate effects of HNF4A2 and HNF4A8 on HNF4A P1 and P2 promoter activities in Ad293 cells.

Data are represented as mean ± SD of n = 4 independent experiments. **p < 0.01 versus GFP control unless otherwise indicated by Student's t test. See also Figure S5.