Skip to main content
. 2019 May 18;17:24–37. doi: 10.1016/j.omtn.2019.05.003

Figure 5.

Figure 5

si-hVDAC1 Treatment Markedly Altered Transcription Factor Expression in U-87MG Cells after the Third and Fourth Transfections

(A and B) Immunoblot (A) and quantitative (B) analyses of p53 levels in protein extracts from si-NT- or si-hVDAC1-treated U-87MG cells after the indicated number of transfections. (C) Real-time qPCR analysis of mRNA levels, at the indicated transfection, of p53, c-Myc, and HIF-1α, using specific primers. The fold change in mRNA levels in si-hVDAC1-treated U-87MG cells, relative to those in si-NT-treated U-87MG cells, is presented. (D and E) Immunoblot of Cyto c in si-hVDAC1-treated U-87MG cells at the indicated transfection (D). Real-time qPCR analysis of Cyto c levels in si-hVDAC1-treated U-87MG cells, relative to those in si-NT-treated U-87MG cells (E). Results reflect the mean ± SEM, *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001.