Table 1.
Summary of the Expression Levels of Genes Associated with Metabolism, Stem Cells, Differentiation, and Transcription Factors as a Function of the Number of Transfections of U-87MG Cells with si-VDAC1, Relative to Transfection with si-NT (qPCR Results)
| Gene |
Fold of Change si-NT/si-VDAC1 |
|||
|---|---|---|---|---|
| Transfection No. | 0 | 2 | 3 | 4 |
| Metabolism | ||||
| Glut-1 | 1.00 ± 0.03 | −1.2 ± 0.13 | −2.90 ± 0.21 | −3.1 ± 0.23 |
| HK-I | 1.00 ± 0.01 | −1.27 ± 0.31 | −1.5 ± 0.11 | −4.4 ± 0.21 |
| LDH-A | 1.00 ± 0.01 | −1.2 ± 0.28 | −1.4 ± 0.08 | −3. 2 ± 0.17 |
| CS | 1.00 ± 0.01 | 1.0 ± 0.14 | −1.6 ± 0.02 | −2.8 ± 0.22 |
| ATP synthase 5a | 1.00 ± 0.01 | −1.10 ± 0.06 | −1.6 ± 0.22 | −2.5 ± 0.17 |
| VDAC1 | 1.00 ± 0.03 | −2.3 ± 0.2 | −2.8 ± 0.23 | −3.3 ± 0.07 |
| VDAC2 | 1.00 ± 0.13 | 1.2 ± 0.08 | 1.3 ± 0.32 | 1.1 ± 0.04 |
| VDAC3 | 1.00 ± 0.08 | 1.11 ± 0.07 | 0.90 ± 0.11 | 1.2 ± 0.04 |
| Stem Cell | ||||
| Nestin | 1 ± 0.12 | −1.17 ± 0.07 | −1.9 ± 0.3 | −15.6 ± 0.05 |
| KLF4 | 1 ± 0.08 | −1.5 ± 0.03 | −3.9 ± 0.04 | −7.5 ± 0.06 |
| SOX2 | 1 ± 0.09 | −1.2 ± 0.03 | −5.2 ± 0.06 | −5.1 ± 0.03 |
| Nanog | 1 ± 0.01 | −1.2 ± 0.22 | −1.2 ± 0.02 | −3 ± 0.21 |
| Differentiation | ||||
| TUBB3 | 1 ± 0.03 | 1.6 ± 0.32 | 11.2 ± 0.7 | 13.2 ± 0.96 |
| MAP2 | 1 ± 0.02 | 1 ± 0.08 | 2.5 ± 0.37 | 3.2 ± 0.18 |
| GFAP | 1 ± 0.01 | 1 ± 1.25 | 1.9 ± 0.55 | 3.7 ± 0.61 |
| Cyto c | 1 ± 0.06 | 1.4 ± 0.38 | 2.5 ± 0.48 | 5.1 ± 0.28 |
| TFs | ||||
| p53 | 0.9 ± 0.06 | 0.9 ± 0.08 | 1.20 ± 0.08 | 4.70 ± 0.10 |
| c-Myc | 1.0 ± 0.12 | 1.07 ± 0.04 | 1.02 ± 0.05 | −21. 4 ± 0.01 |
| HIF1-α | 1.25 ± 0.12 | −1.17 ± 0.07 | −2.40 ± 0.30 | −13.9 ± 0.08 |