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. 2019 Apr 29;43:238–252. doi: 10.1016/j.ebiom.2019.04.041

Fig. 6.

Fig. 6

Blocking FAO signalling abrogates adipose microenvironment-induced chemoresistance of NKX2–8-deleted EOC cells in vitro. a. Logarithmic combination index plot for the combination of Perhexiline and CDDP in the indicated cells. b. Immunoblotting analysis of p-Akt, Akt, p-mTOR, mTOR, p-S6K, p-BAD, Cytochrome c and activated caspase 3 in the indicated adipocyte-CM-treated EOC cells. α-Tubulin served as a loading control. c. Intracellular GSH level in the indicated adipocyte-CM-treated EOC cells. d. Content of DNA-bound CDDP in the indicated adipocyte-CM-treated EOC cells. e. Relative IC50 value for CDDP in the indicated adipocyte-CM-treated cells using MTT cell viability assays. f. FACS analysis of annexin V staining in the indicated adipocyte-CM-treated EOC cells. g. Quantification of the number of colonies formed by the indicated adipocyte-CM-treated EOC cells. Error bars in Fig. 6c-g represent the mean ± SD of three independent experiments. ⁎P < .05.