TABLE 1.

Ponemah Analysis Attribute settings used for marking R waves in ECG.

Attribute	Mice setting	Rat setting
QRS detection threshold	25%	25%
Minimum R deflection	0.03–0.25 mV	0.3 mV
Maximum heart rate	1500 bpm	1200 bpm
Minimum heart rate*	400 bpm	200 bpm
Peak bias	20%	20%

For QRS detection threshold, 25% of the largest rectified derivative signal within a QRS segment is used for identifying potential R waves. Minimum R deflection is the threshold voltage for R wave detection. Maximum heart rate is a hard cut off that will disregard any R waves in which the heart rate exceeds the maximum heart rate setting. Peak bias indicates whether R waves tend to deflect more positively or negatively from the baseline. A higher percent value for peak bias would instruct the software to prioritize marking the positive portion of the QRS complex as the R wave. ^*Minimum heart rate is not a hard cut off, rather, the software will try to find a missing R wave (by lowering the QRS detection threshold) if the minimum RR is obtained and it doesn’t find an R wave.