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. 2018 Apr;44:127–137. doi: 10.1016/j.cellsig.2018.01.004

Fig. 1.

Fig. 1

Oncogenic RAC1 protects HT-1080 human fibrosarcoma cells from ER-stress. A and B, Relative viability (WST-1 assay) of RD cells (A) and HT-1080 cells (B) after knockdown using pools of four siRNA against the indicated targets followed by treatment with 2 mM DTT for 24 h. Data show relative viability of DTT-treated compared to untreated cells for each siRNA. C–F, Relative viability (WST-1 assay) of RD cells (C and D) and HT-1080 cells (E and F) after knockdown using the indicated individual siRNA followed by treatment with 2 mM DTT (C and E) or 20 μg/ml Tm (D and F) for 24 h. Data show relative viability of ER-stressed (DTT or Tm) compared to unstressed cells for each siRNA. G and H, Representative western blots showing expression of RAC1 and β-actin loading control in RD cells (G) or HT-1080 cells (H) after knockdown using the indicated siRNA oligomers (- = untransfected, C = siCtrl). Western blots are representative of three independent experiments. Bar charts show means ± S.E.M. of data from three independent experiments. * p < .05, ** p < .01, *** p < .001, unpaired t-test comparing to siCtrl, n = 3.