Fig. 2.

RAC1 knockdown in HT1080 human fibrosarcoma cells causes increased caspase activity under ER-stress. A and B, Mean ± standard deviation fluorescence readings from an ELISA-based antibody array measuring cleaved caspase-3 (A) and cleaved PARP1 (B) in lysates from HT-1080 cells transfected with the indicated siRNA oligomers, followed by treatment with 2 mM DTT for 2 h (grey bars) or no treatment (black bars). C, Representative western blots showing expression of PARP/cleaved PARP, cleaved caspase-3, RAC1 and β-actin loading control in lysates from HT-1080 cells transfected with the indicated siRNA, followed by treatment with 2 mM DTT for 0, 2 or 4 h. Western blots are representative of three independent experiments. U/T = untransfected.