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. 2018 Oct;69(4):851–860. doi: 10.1016/j.jhep.2018.05.028

Fig. 3.

Fig. 3

Fluorescence loss in photobleaching shows absence of intracellular exchanges between ER inclusions. (A) FLIP of ZZ- and RR-HLCs transfected with KDEL-GFP: Pre-bleach fluorescence was determined before bleaching. Fluorescence was monitored within equidistant areas (stars) from the area of bleach (red box). Graphs show normalised FLIP plots with pre-bleach intensities set to 100%. n = 5. (B) FLIP of ZZ-HLCs with large inclusions transfected with CytERM. Graph shows normalised FLIP plot with pre-bleach intensities set to 100%. n = 3. (C) 100 μm Z-tack image of ZZ-HLCs. Scale bars: 10 μm. FLIP, fluorescence loss in photobleaching; GFP, green fluorescent protein; HLCs, hepatocyte-like cells.