Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jun 6;10:568. doi: 10.3389/fneur.2019.00568

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Deb-Chatterji, Schuster, Haeussler, Gerloff, Thomalla and Magnus.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

(A) Morphology of circulating endothelial cells (CEC, green) with Dynabeads™ (black) attached after immunomagnetic isolation from peripheral blood of a patient with PACNS displayed under a fluorescence microscope. CECs were isolated by CD 146-coated Dynabeads™ and with a fluorescence-coated (FITC) lectin (Ulex europaeus agglutinin 1, UEA-1) in an endothelial cell specific double-staining method. Afterwards CECs were enumerated in a counting chamber using a fluorescence microscope. (B) FACS plot of expanded T helper cells from a patient with biopsy proven PACNS. CSF cells were expanded in the presence of feeder cells, IL-2 und PHA for 2 weeks and an intracellular cytokine staining for IL-17 and IFNγ was performed.