Table 1.

Antibodies used for flow cytometric analysis of equine cell surface markers.

Antibody	Clone/Isotype	Host species	Target species	Fluorophore	2° Antibody	Company	Dilution for 1° antibody
CD29	TDM29/IgG1a	Mouse	Human	APC	Yes Goat anti-mouse IgG	EMD Millipore	1:100
CD44	IM7/IgG2bb	Rat	Human	FITC	No	Thermo IM7	1:80
CD90	?/IgM	Mouse	Canine, Equine	RPE	No	WSU Monoclonal Antibody Center	1:200
CD105	SN6/IgG1b	Mouse	Human	Alexa 488	No	Bio Rad	1:10
CD45RB	?/IgM	Mouse	Equine	RPE	No	WSU Monoclonal Antibody Center	1:200
CD79α	HM57/IgG1c	Mouse	Human	Alexa 647	No	Bio Rad	1:200
MHCI	cz3/IgG2b	Mouse	Equine	APC	Yes Goat anti-mouse IgG	Giftd	1:100
MHCII	cz11/IgG1	Mouse	Equine	APC	Yes Goat anti-mouse IgG	Giftd	1:200
Isotype control	Corresponding MAB		Target species	Fluorophore		Company	Dilution
IgG1	To CD29		Mouse	APC		Abcam	1:100
IgG2b	To CD44		Rat	Alexa 488		Abcam	1:100
IgM	To CD90		Mouse	PE		Abcam	1:200
IgG1	To CD105		Mouse	Alexa 488		Abcam	1:200
IgM	To CD45RB		Mouse	PE		Abcam	1:200
IgG1	To CD79α		Mouse	Alexa 647		Abcam	1:400
IgG2b	To MHCI		Mouse	APC		Abcam	1:100
IgG1	To MHCII		Mouse	APC		Abcam	1:100

^aValidated by Laval et al. (28).

^bValidated by Paebst et al. (29).

^cValidated by De Schauwer et al. (30).

^dGifts from Dr. Doug Antczak, Cornell University, Ithaca, New York, USA.